Composite
NosZ+SYFP

Part:BBa_K1866001:Design

Designed by: Stefan Oehler   Group: iGEM15_IIT_Delhi   (2015-09-16)


NosZ gene+SuperYellowFluorescenceProtein (in pSB1A3)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
    Illegal NgoMIV site found at 908
    Illegal NgoMIV site found at 1072
    Illegal AgeI site found at 282
    Illegal AgeI site found at 1381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1638
    Illegal BsaI.rc site found at 667
    Illegal BsaI.rc site found at 1312


Design Notes

None. This part is compatible with the 3A assembly.


Source

The gene NosZ was isolated from Pseudomonas aeruginosa genome. (BBa_K1356006)

SYFP sequence was obtained from the distribution kit.

References